| 초록 |
A novel polylactide(PLA) and poly(lactic-co-glycolic acid) (PLGA), heparin conjugates, respectively has been prepared, which can be applied as a blood and tissue compatible biodegradable materials in implantable medical devices and tissue engineering. PLA and PLGA were coupled with heparin by N,N-Diisopropylethylamine /O-Benzotriazol-1-yl-N,N,N,N- tertra-methyluronium hexafluorophosphate or N-(dimethylamino) pyridine(DMAP)/dicyclohexyl carbodiimide(DCC). FT-IR, 1H-NMR, elemental analysis and colorimetric assay have confirmed the synthesis of PLA-heparin and PLGA-heparin. PLA-heparin and PLGA-heparin coated surface has shown the lower static contact angle(higher hydrophilicity) than PLA and PLGA surface. In-vitro, platelet adhesion and protein adsorption study revealed that lower platelet and protein adhered on PLA-heparin surface than PLA surface. In addition, the PLA-heparin prolonged the clotting time(APTT) significantly as compared to PLA surface, which might be due to the bound heparins bioactivity. Cell compatibility has been studied from cell attachment test using fibroblast and chondrocyte. Obtained results suggest PLA-Heparin and PLGA-heparin will be utilized as coating and additive materials to improve blood and cell compatibility, especially in scaffold materials in cardiovascular tissue engineering.
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