| 초록 |
For decades, developing protein chips is drawing huge interests for high-throughput biomedicine discovery. However, the site-specific and covalent attachment of proteins on substrate with full retention of their function is still challenging. Adopting thiol-ene click reaction can be a one of solutions because thiol in cysteine can be used as a specific binding site for attaching proteins. However, the preparation of vinylated substrates for attaching cysteine was highly dependent on the substrate materials. Herein, we present a simple surface modification method for preparing vinylated substrates. We used initiated chemical vapor deposition (iCVD) to deposit vinyl-functional polymer, poly(tetravinyl-tetramethyl cyclotetrasiloxane) (pV4D4). The pV4D4 was even applicable to plastics and papers and thiol-ene click reaction was successfully applied to immobilize various kinds of cys-tagged proteins including fluorescent protein and artificial antibody like proteins. |
