| 초록 |
Molecularly imprinted polymers were synthesized by an in-situ therm-initiated copolymerization process in water-containing systems using ciprofloxacin as template, methacryclic acid as monomer, and ethylene glycol dimethacrylate as cross-linker. Recognition properties, binding capability, and chromatographic applications of the molecularly imprinted polymers were investigated and the results revealed the molecularly imprinted polymers have high affinity to template in both in nonpolar and aqueous environment and the affinity can be easily controlled by adjusting the mobile phase. The obtained polymers were successfully used as selective chromatographic stationary phase to extraction and separation of ciprofloxacin from biological samples. After centrifugation, the samples could be directly injected into the imprinted column, proteins and other biological matrix were quickly washed out and the analyte could be selectively retained and enriched. This expedient greatly simplified the overall procedure, resulting in a rapid and efficient sample analysis while maintaining precision and accuracy. |
