| 초록 |
In this study, alcohol dehdyrogenase(ADH) was immobilized on the polyaniline magnetic nanoparticles(PAMP). During the experiments, we found that using the glutaraldehyde as a cross-linker, no acitivity was found in ADH immobilized on the PAMP. However using EDC, immobilized enzyme shows great activities. The Kcat value of the free and immobilized enzymewere detected as 82, 3.4 mM/min/mg protein respectively. And immobilized enzyme shows a good property at the acidic solution. At the acidic condition, free ADH couldn't be activated, however, immobilized ADH shows more than 50% activities at pH 4.5.
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