| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Effect of Keratin on Proliferation and Phenotype of OECs onKeratin/PLGA Scaffold |
| 초록 |
Keratin is the major structural fibrous protein such as wool, hair, and nail and is useful as natural proteinous. Olfactory ensheathing cells (OECs) can stimulate tissue sparing and enhance outgrowth of both intact and lesion axons. We were prepared keratin/PLGA (0, 20 and 50 wt%) scaffolds by solvent casting/salt leaching method. Celluar viability and proliferation were assayed by MTT test. Morphology of celluar adhesion were confirmed by scanning electron microscope (SEM) and RT-PCR was conducted to confirm mRNA expression of NF, NSE and s-100 for OECs marker. In MTT assay results, cell viability in scaffolds impregnated 20 wt% of keratin were higher than other scaffolds. SEM exhibited normal spindle-shaped morphology on 20 wt% of keratin. These results suggest that keratin provide suitable surface to neural cells and proper content affect on culture condition to improve cell adhesion and proliferation. Acknowledgements This research was supported by SCRC(SC3100). |
| 저자 |
오아영1, 김순희1, 정수현1, 김윤태2, 홍현혜2, 전나리2, 강길선2
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| 소속 |
1전북대, 2전북대하교 |
| 키워드 |
keratin; OEC; PLGA
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| E-Mail |
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