| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.444 |
| 발표분야 |
생물화공 |
| 제목 |
Engineered Proteinticles for Targeted Delivery of siRNA |
| 초록 |
Here we genetically engineered human ferritin based proteinticles to simultaneously display various functional peptides on the surface of proteinticles: cationic peptide to capture siRNA, tumor cell targeting and penetrating peptides, and enzymatically cleaved peptide to release siRNA inside tumor cell. The polymerized siRNA (poly-siRNA) tightly bound to the engineered proteinticles and formed stable and condensed structure (poly-siRNA-proteinticle complex) without cytotoxicity problem. Furthermore, siRNAs in the condensed complex were effectively protected from endonuclease due to a shielding effect of proteinticles. In the in vitro treatment of poly-siRNA-proteinticle complex, both of the tumor cell targeting and penetrating peptides were important for efficient delivery of siRNA, and the red fluorescent protein (RFP) expression in RFP-expressing tumor cells was notably suppressed by the delivered siRNA with the complementary sequence to RFP mRNA. It seems that the human ferritin-based proteinticle is an efficient, stable, and safe tool for siRNA delivery, having a great potential for application to in vivo cancer treatment. |
| 저자 |
강윤식, 이은정, 조은지, 박동현, 김희태, 김아름, 장영은, 이지원
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| 소속 |
고려대 |
| 키워드 |
Proteinticles; siRNA; tumor; Targeted Delivery
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| E-Mail |
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| 원문파일 |
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