| 초록 |
The purpose of this study is to provide a cartilage therapeutic agent using a cell aggregate-hydrogel-polymer scaffold complex capable of inducing the effective regeneration of cartilage tissue similar to natural cartilage, while retaining high mechanical strength, flexibility, and uniform morphology. We prepared the cell aggregate-hydrogel-polymer scaffold complex including cell aggregates of bone marrow derived differentiated chondrocytes, a fibrin gel matrix, and a porous polymer scaffold, where the cell aggregates are evenly dispersed in the hydrogel matrix, and the resulting hydrogel matrix is immobilized onto the surface of the polymer scaffold while filling up the pores. The cell aggregates in the hybrid scaffolds of fibrin gels and elastic PLCL scaffolds can encourage themselves to differentiate to chondrocytes, maintain their phenotypes and enhance GAGs production and consequently, improve the quality of cartilaginous tissue formed in vitro and in vivo. |
