| 학회 | 한국공업화학회 |
| 학술대회 | 2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 | 19권 1호 |
| 발표분야 | 생체재료_포스터 |
| 제목 | Oleic Acid-Added Embedding Medium for Histological Analysis of Hard Tissue |
| 초록 | For the histological analysis of hard tissue such as bone, various acrylate-based materials have been used as an embedding medium. However, commercial embedding media are expensive, and cutting the embedded block takes a long time. In this study, mixtures of methyl methacrylate (MMA), di-butyl-phthalate (DBP), and oleic acid (OA) were tested for possible application as an embedding medium for large and small undecalcified bone specimens. Mechanical properties were tested in a compressive mode. We investigated the change of hydrophilicity in the sectioned surface by measuring the contact angle depending on the OA. Crystallinity was analyzed using a X-ray diffractometer (XRD). Surface analysis was performed using a confocal laser scanning microscope. To determine the staining efficiency of staining dyes, hamatoxylin-eosin (H&E) and Masson’s trichrome (MT) staining methods were performed for the histological analysis of bone-implant complex. We confirmed that the investigated embedding media showed good properties such as optimal mechanical strength appropriate for cutting the embedded block and proper staining efficiency for histological analysis. Therefore, the MMA/DBP/OA mixtures can be used as an embedding media appropriate for various hard tissues and bone-implant complex. |
| 저자 | 임진익, 김주승, Le Thi Kim Tram, 김응석, 이우걸 |
| 소속 | Dankook Univ. |
| 키워드 | MMA; Oleic acid; Hard tissue |
