| 학회 |
한국공업화학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
8권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Functional characterization of novS and novU involved in novobiocin producer S. spheroids |
| 초록 |
NovU and novS represent C-methyltransferase and dTDP-4-keto-reductase putatively involved in noviose biosynthetic pathway. We have expressed these genes in E. coli and identified the functions of their corresponding products by in vitro enzyme assay. The results indicated that novU encodes a C-methyltransferase and catalyzes the methylation of the NovW product (dTDP-glucose 3,5-epimerase). Similarly, NovS encodes a dTDP-4-ketoreductase and catalyzes the formation of rhamnose utilizing dTDP-4-keto-6-deoxyglucose in the presence of NADH and NovW. Furthermore, the pathway for the biosynthesis of noviose has been confirmed. |
| 저자 |
Thi Thu, Madan Kumar Kharel, Luitz Heide, 김천규, 이희찬, 류광경, 송재경
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| 소속 |
선문대 |
| 키워드 |
Deoxysugar; biosynthesis of noviose; and streptomyces
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| E-Mail |
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