| 초록 |
A nuclear localization signal was non-covalently attached to DNA for the purpose of enhancing transfection efficiencies of non-viral gene carriers. Psoralen, a nucleic acid-intercalating agent was chemically attached to a signal peptide. The conjugate spontaneously intercalated into DNA and then poly(ethylene imine) [PEI] was added to prepare a DNA/PEI complex containing the signal peptide moieties. The conjugate was slowly released from the DNA/PEI complex for 24 h, while a burst release was examined when the conjugated was added to DNA without PEI. The complex containing a signal peptide moiety increased transfection efficiencies on COS-1 cells, compared to a mutant signal peptide or a control. Therefore, the psoralen-nuclear localization signal is expected to be a potent transfection enhancing agent without a covalent modification of transgenes. |
