| 초록 |
Mannheimia succiniciproducens, a gram-negative rumen bacterium producing succinic acid, was adapted to medium with high-concentration of succinic acid for acid tolerance. Responses of the adapted M. succiniciproducens strain to succinic acid shock were investigated based on transcriptome analysis. During the investigations, gene expression levels of the adapted M. succiniciproducens strain were compared with a parent strain and the expression patterns after acid shock are classified refer to function of genes. Standing on the transcriptome data, specific genes of M. succiniciproducens are selected as targets of genetic manipulations. To improve tolerance for succinic acid, It was tried that overexpression or knock-out experiments of target genes and finding global regulators for acid resistance. [This work was supported by the Genome-Based Integrated Bioprocess Development Project from the Korean Ministry of Education, Science and Technology (No. 2005-01304). Further supports by LG Chem Chair Professorship, and WCU (World Class University) program through the National Research Foundation of Korea are greatly appreciated.] |
