| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
10권 2호, p.1270 |
| 발표분야 |
공정시스템 |
| 제목 |
Calculation of the Intracellular Metabolic Flux Distribution Using Split Ratio of Diverging pathway |
| 초록 |
Metabolic flux analysis (MFA) has become one of the major tools for understanding physiological states of the cell. However, MFA based on the stoichiometric balances cannot compute the real flux distributions of complex systems, which include parallel pathways, metabolic cycles, and bidirectional reactions. To overcome such drawback, additional constraints are required for MFA. Using 13C tracer technique in combination with direct extracellular flux measurement, we can get information of biochemical network, such as fractional contribution of a pathway to a target metabolite pool. In this work, we have developed MFA tools for the analysis of intracellular fluxes and added a split ratio of diverging pathway to the constraints. As a result, it allows users to construct in silico model considering available labeling information and to obtain more realistic intracellular metabolic fluxes. This work was supported by IMT-2000 project from MOST and MIC, BK21 project form MOE, and Center for Ultramicrochemical Process Systems sponsored by KOSEF. |
| 저자 |
오영균1, 최형석1, 김태용1, 이동엽2, 이상엽1, 박선원1
|
| 소속 |
1한국과학기술원, 2Bioinformatics Research Center |
| 키워드 |
metabolic flux analysis; Isotopomer distributions; metabolic modeling
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
