| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
(생물공학) 생리활성 이차대사산물 생산 최신동향 |
| 제목 |
Promoter engineering of microbial secondary metabolite biosynthetic gene clusters |
| 초록 |
Production of secondary metabolites requires the concerted expression of biosynthetic enzymes that are clustered together in microbial genomes and comprise several biosynthetic operons. As their biosynthesis is a highly energy-demanding process, the transcription of biosynthetic operons is tightly controlled by host transcriptional regulatory systems. Due to the complexity underlying the mechanism of transcriptional regulations, it is often challenging to achieve optimal culture conditions that maximize the production yield of target metabolites. Promoter engineering in combination with synthetic biology tools that enable multiplex engineering of biosynthetic gene clusters might provide a potential solution to this problem. Here, we present the development of a CRISPR/Cas9-based synthetic biology platform that allows the complete refactoring of native transcriptional regulatory systems with an aim to maximize the transcription of biosynthetic operons without affecting cell growth. |
| 저자 |
강학수 |
| 소속 |
건국대 |
| 키워드 |
secondary metabolites; promoter engineering; synthetic biology; CRISPR/Cas9
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| E-Mail |
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