| 초록 |
Among numerous challenges faced in the commercial cultivation of microalgae, providing a cost effective water and nutrients source is crucial. Our study aimed to utilize the municipal wastewater as nutrient feedstock for mixotrophic microalgal cultivation by using glycerol as carbon source.In present study, nutrient removal from municipal wastewater by Chlorella vulgaris and Nannochloropsis oculata was investigated by using mixotrophic cultivation with glycerol (0 to 5 g L-1). Performance parameters were assessed by estimating the removal of total nitrogen, total phosphorus, biomass growth, chlorophyll content, lipid yield and fatty acids. With the addition of 2 g L-1glycerol, a maximum biomass productivity of 0.056 g L-1d-1 was achieved in the mixotrophic culture of C. vulgaris within 12 days. The mixotrophic culture showed a 30 fold increase in biomass productivity compared to the photoautotrophic culture. However, the highest total nitrogen removal (80.62%) and total phosphate removal (60.72%) was observed in the N. oculata culture supplemented with 3 and 5 g L-1 glycerol respectively. These results suggest that mixotrophic cultivation using glycerol offers great potential in the production of renewable biomass, waste water treatment and consequent production of high-value microalgal oil. |
