| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
대학원생 구두발표 |
| 제목 |
Co-delivery of BMP2 and SOX9 to recover chondrogenic property of dedifferentiated chondrocyte |
| 초록 |
Bone morphogenic protein (BMP) is an important signal to upregulate osteogenesis and chondrogenesis of stem cell. SRY-related HMG-box gene 9 (SOX9) was reported as the key transcription factors of chondrogenesis. We hypothesized that the co-delivery of the BMP2 and SOX9 genes would render dedifferentiated chondrocyte to recover normal condrogenic properties efficiently. To aim this, we constructed a bicistronic vector encoding the BMP2 and SOX9 genes linked to the 2A peptide sequence. Therefore we confirmed above 70% transfection efficiency of BMP2 and SOX9 gene by RT-PCR and western blot analysis. In RT-PCR analysis and alcian blue staining, expression of chondrogenesis-related markers and GAG matrix formation were significantly increased when BMP2/SOX9 was delivered simultaneously compared to BMP2 or SOX9 gene. These findings demonstrate that the chondrogenic lineage-determining two genes would be able to enhance chondrogenic re-differentiation of dedifferentiated chondrocyte. |
| 저자 |
차병현, 이석준, 강선웅, 이수홍
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| 소속 |
차의과학대 |
| 키워드 |
Chondrocyte; BMP2; SOX9; 2A; Chondrogenesis; Re-differentiation
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| E-Mail |
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