| 학회 | 한국공업화학회 |
| 학술대회 | 2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 | 8권 2호 |
| 발표분야 | 정밀화학 |
| 제목 | Synthesis of Core-Shell Type PEG Grafted PS Supports for Protease-Catalyzed Dipeptide Synthesis |
| 초록 | The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. In the last few years much attention has been paid to protease-catalyzed peptide synthesis by utilizing the reverse pathway of the hydrolytic reaction. But organic co-solvents and highly concentrated substrate suspensions must be used to shift the equilibrium toward the synthesis because the synthesis of amides from free amines and carboxylic acids is thermodynamically unfavorable in bulk aqueous media. We have developed water-compatible and enzymes-accessible core-shell type solid supports which have poly(ethylene glycol) skin layer of various chain length and density. PEG 500, 800 and 1900 were used for evaluating PEG length effect and lysin dendrimers were used for evaluating the PEG density effect for the enzyme activity. Several dipeptides were synthesized by the enzyme-immobilized resins, and analyzed by HPLC. We could confirm the effects of PEG length and density on the activity of resin bound enzyme in dipeptide synthesis. |
| 저자 | 이규택, 이상명, 이윤식 |
| 소속 | 서울대 |
| 키워드 | Protease; peptide; solid support; PEG; water-compatibility |
