| 초록 |
To construct artificial organs, multiple cells need to be assembled and communicate with each other. In this work we employed DNA-PEG-lipid as a linker to assemble neuronal and gustatory cells with aiming to generate a tongue tissue. DNA-PEG-lipid was made of α-N-hydroxysuccinimidyl-ω-maleimidyl poly(ethylene glycol), 1,2-dipalmitoyl-glycerol-3-phosphatidylethanolamine, and two thiol modified single stranded oligonucleotides: oligo(dA)20-SH and oligo(dT)20-SH. Hippocampal neuronal cells from rat embryos and gustatory cells from 2 days neonatal mouse were seeded on a poly-D-lysine coated culture slide. The cultured neuronal cells and gustatory cells were incubated with each complementary DNA-PEG-lipid, followed by simple mixing of the cells for self-organization into gustatory cells on neuronal cells. This work was supported by Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-IT1409-10. |
