| 초록 |
Polyamidoamine (PAMAM) dendrimers are well known non-viral vectors for gene delivery application. Moreover cystamine core PAMAM has ability to degrade inside low pH of endosome leading to the easily release of biomolecules. In present work, we designed γ-amino butyric acid (GABA), histidine, arginine modified cystamine core PAMAM. GABA has four carbon chains that plays a role as flexible linker, hence easy interaction with cell membrane. cPAMAM-GABA-HR synthesis was confirmed by H1-NMR. We checked gel retardation assay and DNA release phenomenon of performed polyplex in low pH using DTT. Cytotoxicity was examined by WST assay and transfection efficiency was confirmed using luciferase reporter plasmid in HeLa cells. GFP expression was detected by fluorescence microscopy. This work can be a promising candidate of non-viral vectors in gene delivery application. |
