| 초록 |
Due to the increasing price and the environmental issue caused by petroleum-based fuels, biodiesel has attracted significant attention as a renewable, non-toxic and environmental-friendly alternative fuel. Conventionally, the synthesis of biodiesel has been accomplished by alkali- or acid-catalyzed transesterification using acyl acceptor such as methanol. In this study, we prepared fatty acid methyl esters (FAMEs) from microalgae by lipase-catalyzed in situ reactive extraction using dimethyl carbonate as reaction medium, extraction solvent and acyl acceptor. This one-pot reactive extraction method produced simultaneously glycerol-free biodiesel and glycerol carbonate directly from microalgae biomass. Various reaction conditions (DMC/biomass ratio, water content, enzyme loading, reaction temperature and time) were analyzed and optimized for an efficient production of FAMEs. |
