| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1804 |
| 발표분야 |
생물화공 |
| 제목 |
In silico Drug Targeting of Vibrio vulnificus CMCP6 |
| 초록 |
Vibrio vulnificus is a halophilic and highly human-pathogenic bacterium, showing very high mortality rate when infected. In order to facilitate the drug development process for this, we undertook in silico analysis to identify specific drug targets in the genome-scale metabolism of V. vulnificus. With a newly sequenced and annotated genome of V. vulnificus, we first reconstructed its genome-scale metabolic network using the KEGG database. Subsequently, we employed constraints-based flux analysis using MetaFluxNet and GAMS to identify essentiality of the genes comprising the metabolic network. Enzymatic reactions whose deletions result in the failure of biomass formation were primarily considered as drug targets. This set of candidates was then compared with the human genome sequence using BLAST to exclude candidates that might cause side-effects in the human body. [This work was supported by the Korean Systems Biology Research Project (M10309020000–03B5002–00000) of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, Microsoft, and IBM SUR program are appreciated.] |
| 저자 |
김태용1, 김현욱1, 정관준2, 김수영2, 이준행2, 이상엽1
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| 소속 |
1한국과학기술원 생명화학공학과 대사공학 연구실, 2전남대 |
| 키워드 |
genome-scale model; constraints-based flux analysis; drug targeting
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| E-Mail |
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| 원문파일 |
초록 보기 |
