| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.422 |
| 발표분야 |
생물화공 |
| 제목 |
Microbial Production of Lactate-Containing Polymers in Metabolically Engineered Escherichia coli |
| 초록 |
Polylactic acid (PLA) has been considered as a good alternative to petroleum-based plastic as it possesses several desirable properties such as biocompatibility, biodegradability, and compostability. For biosynthesis of PLA, we introduced the heterologous metabolic pathways including evolved propionyl-CoA transferase and PHA synthase and engineered based on in silico genome-scale metabolic flux analysis. Several target genes were inactivated and reinforced to redirect flux toward lactate. It resulted in enhanced synthesis of PLA and P(3-hydroxybutyrate-co-LA) in E. coli. In this study, the strategy of combined systems-level metabolic engineering and enzyme engineering allowed one-step production of PLA and its copolymers in E. coli. [“This work was supported by the Technology Development Program to Solve Climate Changes from National Research Foundation of Korea (Development of systems metabolic engineering platform technologies for biorefineries; NRF-2012-C1AAA001-2012M1A2A2026556) and Intelligent Synthetic Biology Center (2011-0031963) of Korea through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST).” ] |
| 저자 |
최소영1, 양정은1, 정유경1, 박시재2, 이상엽1
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| 소속 |
1KAIST, 2명지대 |
| 키워드 |
polylactic acid; E. coli; metabolic engineering
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| E-Mail |
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| 원문파일 |
초록 보기 |
