| 초록 |
In this study, we synthesized a new ratiometric fluorescent peptide using intramolecular CuAAC click reaction in 50% aqueous solution. CuAAC click reaction cause a transformation of intra 1,4-triazole between alkyne and azide of the peptide resulting in enhancement of monomer emission and decrease of excimer emission of pyrene. By using this property, we investigated the changes of reaction rate of CuAAC click reaction under a variety of reaction conditions upon solvent systems, wide range of pH, different copper ion sources, with or without catalytic ligands or reducing agents and the position of azide and alkyne. |
