| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Biodisc Regeneration Using the Gellan Gum Hydrogel |
| 초록 |
The aim of this study is to research effect of gellan gum hydrogel for bio disc tissue engineering on the cell viability and extracellular matix (ECM) secretion. The preparation of gellan gum hydrogel was designed by varying the contents with 1, 2 and 3 wt%. Hydrogels using 1, 2 and 3 wt% of gellan gum are fabricated with cross-linking by EDC (1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide). Cell viability (MTT), glycolsamino-glycan (GAG) assay, histological examination were evaluated to analyze effects of the gellan gum hydrogels on the cell viability and ECM secretion. The gellan gum hydrogels cross-linked by EDC became more increase the rate of cell growth than non cross-linked gellan gum hydrogels. Also the phenotype of the cells maintained better in gellan gum hydrogels having 3 wt% gellan gum contents. These results demonstrated that the gellan gum would significantly affect the cell metabolism. This research was supported by WCU (R31-20029) and MBC (0405-BO010204-0006). |
| 저자 |
유한나, 김하늘, 김형은, 송정은, 송병주, 김영운, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
Gellan gum; hydrogel; disc; EDC(1-ethyl-3-(3-dimethyl aminopropyl)carbodii mide)
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| E-Mail |
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