| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Engineering aldehyde dehydrogenase based on a synthetic selection device to optimize the 3-hydroxypropionic acid production pathway |
| 초록 |
3-Hydroxypropionic acid (3-HP) is an important platform chemical, and biological production of 3-HP from glycerol as a carbon source using glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) has been revealed to be effective because it is a relatively simple metabolic pathway and exhibits higher yield and productivity than other biosynthetic pathways. To apply the directed evolutionary approach to engineer the 3-HP production system, we constructed a synthetic selection device using a 3-HP-responsive transcription factor and developed a selection approach for 3-HP-producing microorganisms. The method was applied to an ALDH library, specifically aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydrogenase (KGSADH). Only two serial cultures resulted in enrichment of strains showing increased 3-HP production, and an isolated KGSADH variant enzyme exhibited a 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild-type one. |
| 저자 |
황현규1, 석주연1, 양진아2, 최상진3, 임현규1, 김경진3, 박성훈4, 유태현3, 정규열1
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| 소속 |
1POSTECH, 2서울대, 3아주대, 4UNIST |
| 키워드 |
synthetic selection device; synthetic biology; 3-HP
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| E-Mail |
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