| 초록 |
Hexanoic acid producing Caproiciproducens galactitolivorans was isolated from sludge of waste water treatment plant. The strain produces hexanoic acid to 90g/L from galactitol in extractive fermentation. In contrast to this, the strain produces lower hexanoic acid in conditions using other cheaper carbohydrates such as glucose. Therefore, in order to investigate genomic characteristics by addition of carbohydrates, the whole genome of this strain was analyzed and RNA expression levels were compared by addition of galactitol and glucose. Genome size and codon sequence number of the strain’s genome were 2,622,912bp and 2,516, respectively. Codon sequences were annotated using genome data base and important genes were investigated. Through this process, two important gene clusters were found. These genes were expressed together in Escherichia coli so as to confirm whether the gene cluster was related to hexanoic acid production indeed. The strain produced hexanoic acid. |
