| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 신진 연구자 특별심포지움 |
| 제목 | Chitosan oligosaccharide-based efficient gene delivery |
| 초록 | Recently, researches on the non-viral gene delivery have focused on the development of efficient gene carriers with excellent safety profiles. Among the various cationic lipids and polymers, chitosan has been considered one of the most promising gene carriers owing to its excellent characteristics such as non-toxicity, biocompatibility, and biodegradability. In this study, to develop chitosan-based efficient gene delivery systems, chitosan oligosaccharides (COS) with various molecular weight (3 ~ 45 kDa) and narrow molecular weight distributions (PDI, 1.1 ~ 1.3) were prepared and their MW-property relationships for gene carrier were studied. The fractionated COSs formed ionic complex nanoparticles with plasmid DNA, showing MW-dependent gene condensations. The in vitro gene transfection revealed that COS-mediated gene deliveries are strongly affected by the MW of COS, COS/DNA ratios, and pH of the transfection medium. The COS9, the most efficient fraction, showed Lipofectamine compatible high gene transfections at the carrier/DNA weight ratio of 30 in the transfection medium pH of 6.5 or below. As second generation COS-based gene carriers, the COS (MW 3 kDa) was chemically modified by bile acids with different hydrophobicities (cholic, deoxycholic, and lithocholic acids). The bile acids modified COS (COSBAs) formed self-aggregated nanoparticles in aqueous milieus and their physicochemical properties were strongly affected by kind of bile acids and degree of substitutions. Among them, COS3CA20 mediated excellent gene transfections (similar to Lipofectamine) with negligible cytotoxic effects. Furthermore, the COS3CA20 mediated gene delivery did not reduced in the presence of 10 % FBS. Therefore, with their excellent safety profiles, fractionated COS (COS9) and COSBA (COS3CA20) may be considered potential candidates for efficient gene carriers. This work was supported by National Research Laboratory (NRL) project of the Ministry of Science and Technology in Korea. |
| 저자 | 채수영, 나재운 |
| 소속 | 순천대 |
| 키워드 | chitosan; molecular weight; gene transfection; bile acids |
