| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Synthesis of β-Amino acid Using A Novel ω-Transaminase and Product Inhibition Studies |
| 초록 |
In these studies, we identified sixteen potential ω-TAs by BLAST and phylogenetic tree analysis. These ω-TAs were cloned, purified and tested for their reactivity for the synthesis of model β-amino acid, a key precursor for sitagliptin. In an enzymatic cascade, lipase converted β-ketoester substrate to β-keto acid, which was subsequently aminated by the selected ω-TA to its corresponding β-amino acid. pH dependency of the product inhibition suggested that lowering the reaction pH to 7.0 can circumvent the inhibition of ω-TA by β-amino acidand about 92.3% conversion of 100 mM β-keto ester substrate could be achieved. The results presented in these studies exhibit an efficient catalytic route for the synthesis of β-amino acids from β-ketoesters and simple pH adjustment as a glimmer for the long-lasting hurdle of product inhibition in ω-TA-mediated biocatalysis. |
| 저자 |
김건희, taresh khobragade, 전현우, 원유미, 성시형, 윤형돈, mahesh patil
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| 소속 |
건국대 |
| 키워드 |
ꞷ-transaminase; β-amino acids; product inhibition; lipase; phylogenetic tree; Sitagliptin precursor
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| E-Mail |
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