| 초록 |
Macrophage-targeted drug delivery is an efficient way to enhance medicinal effects on immunological and infectious diseases. Here, poly(ethylene glycol)/schizophyllan (PEG/SPG) hybrid microgels were fabricated for a specific delivery to macrophages. A precursor solution containing 4-arm PEG-norbornene, dithiol-cross-linker, and photo-initiator was emulsified in dextran and micro-droplets were formed. After shinning UV, PEG microgels of 1.8 μm in diameter were obtained via thiol-ene photo-click reaction. PEG/SPG hybrid microgels were formed via the second UV irradiation with SPG-norbornene. The hybrid microgels successfully entered macrophages (RAW264.7). Whereas much lower number of microgels were uptaken by RAW264.7 pre-treated with SPG solution. These results indicated that the internalization of microgel into the cell was mediated by SPG binding receptors. In BSA delivery test, the cumulative BSA release amount increased with time progress and the BSA was detected in the cytosol. |
