| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | In vitro Neurogenesis of Bone Marrow Stromal Cells on SIS Sponge |
| 초록 | Bone marrow stromal cells (BMSCs) have pluripotent differentiation potential including differentiation into osteoblasts, chondrocytes, adipocytes, and muscle cells.1 Meanwhile small intestimal submucosa (SIS) is a naturally occurring tissue matrix composed of extracellular matrix proteins and various growth factor. SIS is easily derived from the porcine jejunum. Therefore, SIS have the potential in the biomedical application as the sponge. In this work, we examined the differentiation of rat bone marrow stromal cells (rBMSC) into neurocytes on SIS sponge in the presence of neurogenic media. SIS solutions of 2 wt% with 0.1% pepsin in 3% acetic acid were reacted for 48 hrs and freeze-dried. They were treated with 1-ethylene-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) of 50 mM for 24 hrs and lyophilzed. rBMSCs were supplemented with DMEM and 10% FBS, cultured for up to passage 8. Then the rBMSCs were cultured on SIS sponge. For in vitro neurogenesis, rBMSCs were supplemented with pre-induction treatment for overnight. And then neuronal differentiation was performed with DMEM, 2% DMSO and 200 μM BHA for 5hr. The long-term neuronal medium consisted of DMEM, 2% DMSO, 200 μM BHA, 25 mM KCl, 2 mM valproic acid, 10 μM forskolin, 1 μM hydrocortisone, 5 μg/ml insulin. While, control medium consisted of DMEM, 10% FBS and 1% penicillin-streptomycin. rBMSCs on sponge maintained for 5hs, 1, 3, 7 and 14 days. Neurogenesis differentiation in vitro was evaluated by means of SEM, MTT, RT-PCR, and immunohistochemistry. Cell viability on sponge increase as culture day increased. The results of these experiments indicated that rBMSCs could be differentiated into neurocytes. Acknowledgement : This work was supported by KMOHW (0405-BO01-0204-036). Fig. 1. MTT results according to the media Reference 1. F. Sugiura, H. Kitoh, and N. Ishiguro, B. B. R. C. 316, 233 (2004). 2. A. M. Woods, E. J. Rodenerg, M. C. Hiles, F. M. Pavalko. Biomaterials. 25, 515 (2004) |
| 저자 | 홍금덕1, 송인범1, 이민숙1, 김문석2, 조선행2, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | BMSC; SIS sponge; Neurogenesis |
