| 초록 |
The rapid and sensitive detection of bacterial pathogens is crucial for improving patient healthcare. Currently, culture-based assays remain the gold standard in the clinic which involve long procedure times and low sensitivity. We have investigated various molecular labeling approaches which can be robustly applied in clinical settings for detection of the pathogens. We first developed a nanoparticle labeling strategy to ubiquitously detect Gram-positive bacteria using a miniaturized nuclear magnetic resonance device (μNMR). To specifically identify each type of pathogen, we developed a nanoparticle hybridization technology based on detection of bacterial 16S rRNA sequences, allowing rapid and target-specific detection of pathogenic bacteria with extremely high sensitivity. In sum, the molecular sensing strategies based on nanoparticle labeling introduce robust and sensitive platforms for detecting various pathogens and diagnosis of infected patients. |
