| 초록 |
Isolation of targeted cells from peripheral blood has significant applications in cell culture, therapy monitoring and drug development. The extraordinary rarity of targeted cells in the blood stream makes their isolation a significant technological challenge. Herein, electrospun nanofiber composed of poly(ε-caprolactone)-polyethylenimine (PCL-PEI) was modified with various blending ratios of poly(ethyleneglycol)-biotin (PEG-biotin) and PEG. The terminal NHS group of PEG was conjugated with surface exposed amine group on nanofibers and avidin was specific incorporated via biotin-avidin interaction. The incorporated amount of avidin was adjustable according to the content of biotin. When the content of biotin was increase from 0 to 100%, the nanofibers showed a high capture efficiency of the biotinylated cell. Thus, the content of biotin on the surface selective captures targeted cells and interrupts the non-specific adsorption by anti-fouling effect of PEG chains. |
