| 초록 |
Sensitive detection is extremely important in clinical diagnostics, gene therapy, and a variety of biomedical studies. Over the past decades, polymeric nanoparticles have gained increasing attention as optical platforms for sensitive detection of biomacromolecules (DNA, protein and cell) due to the amplification of fluorescent signals. To enhance fluorescent signal, we synthesized fluorescent random copolymer by radical polymerization, which consisted of three parts: a fluorescent group and functional group, to which biomolecules were conjugated and to stabilize the nanoparticles. Fluorescent nanoparticles were developed by nanoprecipitation. The nanoparticles are highly fluorescent, extremely stable, and easy for immobilization for biomolecules. The ultrasensitive property of fluorescent nanoparticles was examined by measuring fluorescence intensity based indirect immunoassay using bovine serum albumin(BSA) and Anti-BSA. |
