| 학회 | 한국공업화학회 |
| 학술대회 | 2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 | 19권 2호 |
| 발표분야 | 생물공학_포스터 |
| 제목 | Simplified and cost-effective cell-free protein synthesis system using continuous-exchange bioreactor. |
| 초록 | Cell-free protein synthesis (CFPS) is a powerful tool for rapid production of recombinant proteins as well as for functional analysis of genes. The unique flexibility and throughput of cell-free protein synthesis have made this technology a promising alternative to conventional cell-based protein production methods. Despite the versatility of CFPS, however, it requires further improvements in protein productivity. One of the measures for improving yield is a continuous-exchange cell-free (CECF) system. The CECF system supports extended syntheses of target proteins through continuous supply and removal of substrate materials and by-products, respectively. However, previous CECF platforms have suffered from the complexity of reaction devices and unacceptably high reagent costs. In this study, we tried to simplify CECF system with cost-effective substrates. First, we designed a simplified CECF device using common lab materials. In addition, we prepared a concentrated master mix solution to simplify the reaction schemes. Finally, nucleoside triphosphates (NTP) were replaced with nucleoside monophosphates (NMP) that are far more cost-effective. These simplified CECF platform with cost-effective substrates led to a significant increase yield of protein synthesis with a fraction of the cost required for conventional CECF reactions. |
| 저자 | 김동명, 권진호, 이경호 |
| 소속 | 충남대 |
| 키워드 | Cell-free protein synthesis; Recombinant protein expression; Continuous exchange cell-free system |
