| 초록 |
In this work, we attempted a sequential expression of different proteins in a same reaction mixture for cell-free protein synthesis. While a cell-free protein synthesis system of the conventional batch configuration could support translation reactions only for limited time periods and protein synthesis stops within an hour, the translational activity in a completed reaction was recovered by dialyzing the reaction mixture against an appropriate buffer. As a result, when the reaction mixture was re-programmed with second DNA template, the encoded protein was successfully generated by using the ‘recycled’ translational machinery. We expect that the proposed approach will offer a facile route to the preparation of protein pairs for studying the structure and function of interacting proteins. |
