| 초록 |
Kappaphycus alvarezii is a rich source of structurally diverse bioactive compounds with various important industrial applications. Recovery of these sulfated galactans has been successful with different conventional technologies. Hence, an alternative extraction process using sub-critical water extraction (SWE) is being successfully carried out for extracting ĸ-carrageenan from its natural bio resources. The SWE is characterized by processing the sample at a temperature ranging from 100oC to 374oC and a pressure up to 22.1 MPa. In this study, we did optimization based on a preliminary range of extraction variables including temperature, pressure, solid-liquid ratio and sonication time using a three level Box–Behnken response surface experimental design on SWE for obtaining the highest kappa carrageenan yield from K. alvarezii seaweed. The results showed that the highest yield of 78.8% crude kappa carrageenan extracted by SWE was at 130oC, 5 MPa, 0.063 mg/L and 10 min sonication time. Thus, the crude kappa carrageenan recovered in the extract was confirmed using FT-IR, TGA and XRD. |
