| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.666 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Measurements and Analysis of Lateral Diffusion of Lipopolysaccharide on Model Bio-Membrane using Fluorescence Tracking (CLSM) Method |
| 초록 | LPS (lipopolysaccharide) of Gram negative bacteria cell membrane, as a natural oligomer, is known to induce initial immune response and play important roles leading to the endotoxic sepsis shock when it streams in human body. But in view of an initial molecule interaction has been not clear up, there is a limitation to account for a sepsis phenomenon. As a fundamental study of single molecule interaction of LPS, observation mobility changes of LPS diffusion behavior on membrane while reaction with extra proteins, we emboded bio-mimetic LPS membrane on mica substrate by LB method. Using FRAP (Fluorescence Recovery After Photobleaching) technique of CLSM (Confocal Laser Scanning Microscope)1, we treated ROI (Region of Interest) photobleaching selectively. As a result of this experiment, we acquired quantification values via LPS diffusion mechanism (charactierization time(tD),mobile fraction(R), diffusion coefficient(D))2. Supplementally, To examine into change of membrane rigidity, we embeded cholesterols of various ratio into model bio membrane and confirmed. To verify of lipid layer properties, we used IR, XPS, AFM image analysis, X-ray scattering. Fig.1 When a region in the fluorescent area is bleached at time t0 the fluorescence decreases from the initial fluorescence . The fluorescence recovers over time by diffusion until it has recovered . The characteristic diffusion time tD indicates the time at which half of the fluorescence has recovered. 참고문헌 1. T.Jean-Francois. Laurence Dupou, Andre Lopez, Prog..Lipid. Res, vol 3, 203(1994) 2. Martha Triantafilou, Siegfried Morath, Alan Mackie, J. Cell. Sci. Vol 117, 4007(2004) |
| 저자 | 장순남, 김종수, 김의숙, 김성수, 조길원 |
| 소속 | 포항공과대 |
| 키워드 | LPS. model biomembrane; CLSM; FRAP |
