| 초록 |
Heparin has exerted a wide range of biological functions though the interactions with enzymes and growth factors. Since heparin is also known to possess TGF-β1 binding site, it has found a variety of applications in the areas of tissue engineering. In this study, non-porous poly(L-lactic acid) (PLLA) films and dual pore PLLA scaffolds were prepared using a solvent casting and gas foaming methods. Their hydrophobic surfaces were then activated using argon plasma treatment, leading to the surface-grafting of acrylic acid. The resultant carboxyl groups were readily reacted in a mild aqueous solution, containing EDC/NHS/MES buffer (pH 5.6), followed by the grafting of heparin. The surface properties of the modified PLLA substrates were analyzed by ATR-FTIR, ESCA, SEM, Toluidine blue O, and contact angles measurement. Once adipose-derived stem cells were seeded onto the scaffolds, cell-substrate interactions were examined using various methods, including SEM observation and WST-1 assay. |
