| 초록 |
Xylose is one of the major fermentable sugars present in lignocellulosic biomass, the second most abundant carbohydrate polymer in nature.Therefore, many research efforts have been focused on the screening or developing of xylose-fermenting microbes. For example, native saccharomyces cerevisiae (S. cerevisiae), widely usedfor industrial ethanol production, can not ferment xylose, so thatby genetical introdcution of xylose reductase (XR) and xylitol dehydrogenase (XDH) into S. cerevisiae, xylose-utilizing recombinant yeast has been developed. However, still efficient xylose-fermentaion has not yet been achieved which isrequired to develop economically viable processes for producing biofuel, ethanol, from biomass. In this study, we employed two kinds of rational design strategy for XR and XDH;(1) cofactor-preference design, (2) thermostability-increase design.When recombinat S. cerevisiae expressing thenewly-designed XR and XDHwas employed for xylose fermentation, efficeint ethnaol production was achieved. |
