| 초록 |
13C-MFA (Metabolic Flux Analysis) is a technique for decoding fluxes of central carbon metabolism using a C source marked 13C. Provide a C source marked 13C in strain and measure the label information of the metabolite. It is then possible to track label information based on a model that decodes flux using specific enzymes in the strain. Resveratrol is a beneficial compound found in plants. It has the effects of cancer, antioxidants and life extension. Resveratrol can be created using Acyl-CoA in the EMP pathway and Tyrosine in the PP pathway. However, the road has a different temper and is difficult to turn into a single strain. Thus, an attempt was made to produce resveratrol using a coculture. In this study, two E. coli were designed to supply more resveratrol precursor based on previous studies. Then we will produce resveratrol with a coculture. Finally, using 13C-MFA to analyze the metabolic rate of coculture, and ways to increase resveratrol will be discussed. |
