| 초록 |
Our research goal is to identify mutants of E. coli GlnRS that can utilize the keto-containing glutamine analogue 2-amino-5-ketohexanoic acid (wherein the amide side chain of glutamine is replaced by a methyl ketone). The keto group can undergo a variety of reactions with hydrazides, alkoxyamines, and semicarbazides under physiological conditions. It can be used to produce proteins modified with fluorescent group, biotin, polyethylenglycol, saccharide and so on. To genetically encode 2-amino-5-ketohexanoic acid in yeast, we are working on isolation of the desired mutants of E. coli GlnRS using rational design and random mutagenesis of the active site. |
