| 학회 | 한국화학공학회 |
| 학술대회 | 2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 | 23권 1호, p.499 |
| 발표분야 | 생물화공 |
| 제목 | Development of novel biosynthesis pathway for the production of acrylic acid in Escherichia coli via β-alanine |
| 초록 | Acrylic acid is an important industrial chemical, which is widely used in coatings, polymers and adhesives. Currently, most of acrylic acid is produced by oxidation of propylene, which causes immense CO2 emission. To replace petroleum-based process, production of acrylic acid from renewable resources is very important. Herein, we report a novel biosynthetic route to produce acrylic acid from glucose via β-alanine, β-alanyl-CoA, and acrylyl-CoA. To verify the pathway, in vitro enzyme assay was carried out. The designed pathway was introduced into Escherichia coli to produce acrylic acid from β-alanine. After the verification of designed pathway in vivo and in vitro, constructed pathway was introduced into β-alanine producing E. coli strain. The resulting strain could produce acrylic acid from glucose. This is the first report to produce acrylic acid via B-alanine route. (This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557)) |
| 저자 | 고유성, 김제웅, 채동언, 송찬우, 이상엽 |
| 소속 | KAIST |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
