| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Antibiotics-free Stable and Tunable Maintenance of Multi-copy Plasmid by Auxotrophic Control |
| 초록 |
Plasmids require addition of antibiotics for their stable maintenance despite the growing concerns over economic and environmental problems. Here, we reinvented the auxotrophic plasmid maintenance system for stable and tunable gene expression during antibiotic-free cultivation. We expressed one of essential genes, infA encoding translation initiation factor (IF-1), based on plasmid rather than chromosome. With this, the gene expression was stably maintained for 5 days with minimized cell-to-cell variation. Moreover, plasmid copy number (PCN) was rationally tunable in 5-fold by varying expression of infA. To demonstrate its usability, the strategy was applied in metabolic engineering for chemical production. Notably, PCN control enabled significantly improved production of both itaconic acid (160%) and lycopene (278%) compared to cultivation with antibiotics. Collectively, the developed strategy could be a platform for production of value-added products in antibiotic-free cultivation. |
| 저자 |
강채원1, 정규열1, 임현규1, 양진아2, 노명현1, 서상우2
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| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Plasmid; PCN maintenance; PCN regulation; Essential gene expression level
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| E-Mail |
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