| 학회 | 한국화학공학회 |
| 학술대회 | 2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 | 16권 2호, p.2428 |
| 발표분야 | 대사공학 신기술 동향 심포지엄 |
| 제목 | Engineering intelligent Escherichia coli to sense and remove heavy metals |
| 초록 | Synthesis of intelligent genetic network in bacteria is one of the great applications of synthetic biology.Here we show the assembly of an intelligent system that can trigger bacteria to sense and respond to the removal of heavy metals such as zinc and copper by engineering of two component systems. This bacterial system can sense zinc and copper using OmpR and NtrC family of two component sensor system and remove metals contaminants through cell surface display system. The genetic circuit of HydHG and CusSR were fused to cell surface display system for the expression of chimeric protein namely OmpC with heavy metal binding peptide. Our Intelligent bacterial system targets the specific heavy metal and triggers a cell surface display system in response to the heavy metal added to the bacterial population which is potentially used in the field of biosensor and bioremediation. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea] |
| 저자 | Sambandam Ravikumar, 유익근, 홍순호 |
| 소속 | 울산대 |
| 키워드 | Synthetic biology |
| 원문파일 | 초록 보기 |
