| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
37권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Proliferation and Differentiation of Schwann Cell in SIS Sponge of Various Contents |
| 초록 |
The porcine small intestinal submucosa (SIS) consists of some growth factors which can stimulate cell activity without immune rejection responses. Thus, SIS had been widely used as a biomaterial. We evaluated the effect of content SIS sponge on proliferation and differentiation of schwann cells (SC). SCs were harvested from the femurs and tibias of female Fischer rat. SIS sponges were prepared by freeze-drying method from 1, 2 and 3 wt% SIS solution. The effect of SIS sponge on the proliferation and differentiation of SCs was assessed in culture using the MTT assay, SEM, swelling test. RT-PCR was conducted to confirm mRNA expression of S100, NSE and NF for nerve marker. In addition, immunochemical evaluation for cellular proliferation and differentiation by H&E and anti-NF staining. Theses studies revealed that SIS sponge has a positive effects on the growth of SCs and the phenotype was maintain through the study. This research was supported by WCU (R31-20029) and SCRC (SC4110). |
| 저자 |
이정환, 심초록, 고현아, 송정은, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
Schwann cell; SIS; Sponge
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| E-Mail |
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