| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 |
15권 1호, p.915 |
| 발표분야 |
재료 |
| 제목 |
Highly Efficient Cholesterol Biosensor Based on Low-Temperature Synthesized ZnO Nanoparticles |
| 초록 |
We report here an easy and successful synthesis and detailed characterization of Zinc oxide nanoparticles by simple solution process at ~90 0C by using zinc acetate, LiOH, and HMTA. The detailed structural characterizations exhibited the nanocrystalline nature of as-synthesized products. A high-sensitive cholesterol amperometric biosensor based on the immobilization of cholesterol oxidase (ChOx) onto as-synthesized ZnO nanoparticles has been fabricated which shows a very high and reproducible sensitivity of 23.7 AmM-1cm-2, detection limit (based on S/N ratio) 0.37 ± 0.02 nM, response time less than 5s, linear range from 1.0 ~ 500.0 nM and correlation coefficient of R = 0.9975. A relatively low value of enzyme’s kinetic parameter (Michaelis-Menten constant) ~ 4.7 mM has been obtained which indicates the enhanced enzymatic affinity of ChOx to Cholesterol. To the best of our knowledge, this is the first report in which such a very high-sensitivity and low detection limit has been achieved for the cholesterol biosensor by using ZnO nanostructures modified electrodes. |
| 저자 |
Mohammad Vaseem1, Ahmad Umar2, 김상훈3, 한윤봉1
|
| 소속 |
1School of Semiconductor and Chemical Engineering, 2and BK 21 Centre for Future Energy Materials and Devices, 3Chonbuk National Univ. |
| 키워드 |
Nanoparticles; Biosensor
|
| E-Mail |
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| 원문파일 |
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