| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
| 권호 |
19권 2호, p.1535 |
| 발표분야 |
생물화공 |
| 제목 |
Structural Analysis of Protein-Aptamer complexes by X-ray crystallography |
| 초록 |
Aptamers are single-stranded DNA or RNA oligonucleotides that bind with high specificity and affinity to a wide range of target molecules including proteins, peptides, and small molecules. Despite numerous advances in the aptamer research, characterization of their specific interactions is often restricted to binding affinity. This void of understanding aptamer-protein interactions can be overcome by x-ray crystallography. Crystal structures of aptamer-target complexes can provide detailed information of their interactions, are therefore crucial for a thorough understanding of the molecular basis of aptamer-target binding mode. The use of advanced protein expression, purification, crystallization screens and powerful synchrotron radiation sources enable high-throughput structure determination, useful for functional analysis Protein-Aptamer complexes. |
| 저자 |
Simranjeet Singh Sekhon1, 이문종1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
ssDNA; protein-aptamer complex
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| E-Mail |
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| 원문파일 |
초록 보기 |
