| 초록 |
The study demonstrates the targeted delivery of doxorubicin hydrochloride to human osteosarcoma cancer cell lines using functionalized dextrin based crosslinked, pH responsive and biocompatible nanogel. The nanogel has been prepared through Michael-type addition reaction. The structure, composition, morphology of the nanogel have been explored using diverse analyses. The TEM analysis confirmed that the size of nanogel appeared within 100 nm, while DLS study indicates that the diameter of the nanogel remained between 113 and 126 nm. The in vitro cytocompatibility results ascertained that the nanogel is non-toxic to hMSCs. In vitro cellular uptake study confirmed that FITC-loaded nanogel can cross the cellular membrane and be well uptake by the cell cytoplasm. The nanogel could efficiently encapsulate doxorubicin with the loading efficiency of 27% after 72 h, and demonstrates anti-cancer activity towards cancer cells and release the encapsulated drug in a controlled way. |
