| 초록 |
Sequence-specific DNA detection plays an important role in the diagnosis and monitoring the genetic related diseases and fatal virus infections. Although the sequence recognition methods by Watson–Crick base pairing are extensively used for DNA sensing in most laboratories, they are generally required denaturation and/or rehybridization steps because the target DNA strand usually forms a duplex with complementary strand. Here, we report a direct dsDNA detection method using the invasion capability of peptide nucleic acid (PNA) and preferential binding of single-stranded PNA (ssPNA) over DNA/PNA duplex to graphene oxide (GO). Unlike other dsDNA sensors based on complementary DNA probes, PNA in combination with GO enabled the hybridization with the target sequence hidden as a duplex form without denaturing step, and the formation of PNA/DNA duplex was translated into selective fluorescence signal according to the concentration of target. |
