| 초록 |
In the gene delivery system, Poly(amidoamine)(PAMAM) dendrimer has been used as an ideal polymeric carrier. PAMAM dendrimer have branched structure with 64 amine residues and studied for different applications such as drug, imaging agent and gene delivery. In this study, PAMAM Generation 4 -Glycine-Leucine-Phenylalanine-Glycine(GLFG)-Histidine-Arginine was designed for novel gene delivery carrier to enhance transfection efficiency. Oligopeptide GFLG was selectively cleaved by enzyme cathepsin B in the lysosome compartment and His and Arg were modified for proton sponge effect and cell uptake, respectively. PAMAM G4-GLFG-H-R was confirmed to form complex with plasmid DNA at 1:8 (Polymer : pDNA) weight ratio. This polyplexes were measured using dynamic light scattering (DLS). Enzymatic release test shown that complex of G4-GLFG-H-R was collapsed by enzyme Cathepsin B. |
