| 초록 |
A novel surface-modified nanodiamond (ND), polyamidoamine-decorated nanodiamonds (PAMAM-NDs) were prepared for delivering E6 or E7 oncoprotein-suppressing siRNA. It is important to understand the physicochemical Calorimetric and UV, CD, DSC spectral study of siRNA/PAMAM-ND complexes proved that the hydrogen bonding and electrostatic interaction between siRNA and PAMAM-NDs decreased endothermic heat capacity. Moreover, siRNA/PAMAM-ND complexes showed low cell cytotoxicity and significant suppressing effects for target E6, E7 oncogenic genes. The cellular uptake of siRNA/PAMAM-ND complexes indicates their internalization via macropinocytosis or endocytosis followed by endosomal escape. From these results, siRNA/PAMAM-ND complexes provide a potential organic/inorganic hybrid material source for gene delivery carriers. |
